MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.
Micro Elements mg/l μM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 6.20 100.27
KI 0.83 5.00
MnSO4.H2O 16.90 100.00
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 8.60 29.91
Macro Elements mg/l mM
CaCl2 332.02 2.99
KH2PO4 170.00 1.25
KNO3 1900.00 18.79
MgSO4 180.54 1.50
NH4NO3 1650.00 20.61
Vitamins mg/l μM
myo-Inositol 100.00 554.94
Nicotinic acid 1.00 8.12
Pyridoxine HCl 1.00 4.86
Thiamine HCl 10.00 29.65
Total concentration Micro and Macro elements including vitamins: 4414.09 mg/l
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).
了解详情，咨询北京启维益成科技有限公司！ |
免责声明： 以上所展示的信息由会员自行提供，内容的真实性、准确性和合法性由发布会员负责，企业录对此不承担任何责任。如有侵犯您的权益，请来信通知删除。