The BisulFlash™ DNA Modification Kit is a complete set of optimized buffers and reagents to perform DNA modification using a next generation DNA bisulfite conversion technology developed by Epigentek. Through a proprietary composition which allows DNA denaturation and bisulfite conversion to be processed at the same time, the complete procedure is reduced to only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Perfecting Bisulfite Conversion
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step -- but with the BisulFlash™ method, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. This breakthrough approach enables the DNA conversion process to be significantly faster with higher conversion efficiency and accuracy. We continue to innovate with the development of the new BisulFlash™ kit by identifying four critical components of bisulfite conversion:
Speed: Reduce the entire procedure to as short as 30 minutes without any reagent setup time.
Efficiency: Completely convert unmethylated cytosine into uracil -- modified DNA > 99.99%.
DNA Protection: Protect against DNA degradation of which more than 90% of DNA loss can be prevented, allowing for greater recovery.
Sensitivity: Start with the lowest amount of input DNA for modification -- only 0.2 ng or just 50 cells.
The convenient ready-to-use, DNA conversion mix solution and single temperature incubation along with the features mentioned above allow for true perfection in bisulfite conversion. The BisulFlash™ DNA Modification Kit is suitable for bisulfite sequencing, MS-PCR, real time MS-PCR, and methylation microarray.
Comparative Overview of Commercial Kits
Data was obtained through actual use of the kit, customer feedback, or information provided by the supplier's datasheet or website.
了解产品详情，咨询北京启维益成科技有限公司！ |
免责声明： 以上所展示的信息由会员自行提供，内容的真实性、准确性和合法性由发布会员负责，企业录对此不承担任何责任。如有侵犯您的权益，请来信通知删除。